BIOFIR is a product that can rightfully be included in the category of probiotics thanks to the presence of lactobacilli and bifidobacteria balanced according to the right ratio, completed by the addition of Digestive Enzymes, Ovotransferrin and Vitamin D. To be used in all cases of intestinal dysbiosis: antibiotic therapy, diagnostic tests (endoscopy), irritable bowel syndrome, Chron’s disease, Gastric Reflux Diseases.
COMPOSITION:
Bifidobacterium lactis: Provides natural defenses against pathogenic bacteria through the production of locally acting antibiotics, betteriocins. It is also able to reduce intestinal inflammation that occurs during an imbalance of the bacterial flora and restore the integrity of the intestinal mucosal barrier, avoiding the migration of pathogenic bacteria towards the genitourinary tract. It shows particular resistance to bile so it is able to colonize the upper part of the intestine.

Bifidobacterium longum: Provides natural defenses against pathogenic bacteria through the production of locally acting antibiotics, betteriocins. It is highly present during childhood but radically decreases in adulthood. It possesses proven probiotic activities, its presence has been associated with many health benefits including improving lactose tolerance and preventing diarrhea, food allergies and pathogen colonization. Some strains of B. longum have been shown to have an antioxidant effect by inhibiting the peroxidation of linoleic acid, a process that results in the creation of lipid hydroperoxides which decompose into highly reactive radicals associated with aging and diseases related to age. Lactobacillus Rhamnosus: Among the lactobacilli it is certainly the one of greatest interest and among the most studied for its multiple properties. As Bifidobacteriumlactis demonstrates resistance to bile and stomach acid, it is able to prevent infections of the renal tract and genitourinary as well as obviously having a probiotic action in the intestine where it is able to counteract the action of pathogenic bacteria and decrease the duration of diarrhea.

Lactobacillus Plantarum: This bacterial strain is characterized by the ability to transform oxygen into hydrogen peroxide, which is important for fighting numerous harmful germs in the human body, specifically this action is carried out in the intestinal tract.

OvotransferrinOtrf

Ovotransferrin, also called conalbumin, is a compound egg white glycoprotein that has a molecular weight of 76,000 daltons and contains about 700 amino acids.

Ovotransferrin constitutes about 13% of the albumen and among the various protective functions of Otrf, the most important is probably the antibacterial activity, which is directly related to the ability of Otrf to bind iron (Fe3 +), making it unavailable for bacterial growth.

The antiviral activity of Otrf was first demonstrated against avian herpesvirus Marek’s disease virus (MDV). It has been hypothesized that the antiviral activity of ovotransferrin is associated with two bioactive peptide fragments of Otrf capable of blocking the virus infection, DQKDEYELL and KDLLFK.

Otrf also has antioxidant properties by mimicking the action of superoxide dismutase which is responsible for the reduction of superoxide radicals (O2). Otrf-derived peptides showed synergistic antioxidant effects with vitamin C.

Otrf hydrolyzate (obtained using enzymes such as trypsin and α-chymotrypsin) showed protective effects against oxidative stress including DNA damage in human leukocytes.
Among the anti-inflammatory properties of OTRF in birds, it has been scientifically proven that
during inflammation the levels of Ortf in the blood remain elevated as long as the inflammation persists. More recently, two tripeptides, IRW and IQW, both derived from hydrolysis of Otrf, have been found that attenuate TNF-α-induced inflammatory responses and oxidative stress in vascular endothelial cells. Other peptides derived from Ortf hydrolysis have shown immunomodulatory activity against T cells and macrophage stimulating activities in vitro, indicating that they may also be good candidates for pharmaceutical use in humans.

Ortf’s hydrolysis products also have very strong anticancer effects against colon (HCT-116) and breast cancer (MCF-7) as they induce apoptosis on malignant cells. Just as antihypertensive properties have been demonstrated, in fact, a subsidiary antihypertensive function of Otrf was detected in the KVREGT peptide of Otrf showing both an ACE-inhibitory action and a vasodilatory activity

Vitamin D
Vitamin D and its prohormones have been the focus of a growing number of studies that have demonstrated their function not only in calcium metabolism and bone formation, but also their interaction with the immune system. In particular, it modulates the activity of the immune system in the intestine and can influence the composition of the microbiome.

A further favorable role of vitamin D, scientifically recognized only recently, but actually exploited unconsciously for decades in respiratory medicine, concerns the ability of this compound to modulate the activity of the immune system:

– promoting it, when it is necessary to fight infections

– attenuating it, when it is excessive and gives rise to chronic inflammatory or autoimmune diseases.

The recent study by H. Schäffler et Al., Published in March 2018, aimed to investigate whether the administration of vitamin D could have an impact on the bacterial composition of the intestinal flora in the case of patients suffering from Crohn’s disease compared to healthy people. This study showed that the microbial communities present in patients with Crohn’s disease are significantly changed during an initial administration of vitamin D, which was not observed in controls. On the contrary, however, the same study found that a further increase in vitamin D administered corresponds to an inversion of this effect, leading to a decrease in the bacterial richness of the intestinal microbiome.
Bacterial species, classifiable between Firmicutes and Actinobacteria, are significantly increased following the administration of vitamin D only in patients with chronic disease and not in healthy controls. This result demonstrates the fact that this nutrient has a specific influence on various bacterial communities that characterize the intestines of patients suffering from chronic intestinal inflammatory diseases. The administration of vitamin D can therefore have a positive effect in case of Crohn’s disease, modulating the intestinal bacterial composition and also increasing the number of potential beneficial bacterial strains.

According to some recent studies there is a link between colitis and vitamin D deficiency.
Irritable bowel syndrome is not a disease itself, but a malfunction of our intestines. To date the causes of this problem have not yet been clarified, but the symptoms are quite evident. In fact, colitis alters intestinal motility, causing bloating and flatulence, stomach ache and cramps. Those who suffer from colitis alternate between diarrhea and constipation, experiencing a series of symptoms more or less intensely.
Recent studies have highlighted a close link between irritable bowel syndrome and vitamin D deficiency.
Low levels of this substance in the body have already been indicated as causes of inflammatory bowel diseases and colorectal cancer. There may therefore also be a link with irritable colon. This is revealed by research that has shown that vitamin D deficiency is present in the majority of patients suffering from this pathology.

Digestive enzymes
Enzymes are essential for the well-being of the body and the insufficiency of even a single enzyme, can cause serious disorders related to indigestibility and malabsorption
• Proteases – break protein bonds to form shorter amino acid chains
• Amylases – reduce polysaccharides to disaccharides – lactose, maltose and sucrose
• Lipases – break down the bonds of triglycerides, transforming them into individual fatty acids and glycerol
• Cellulase – responsible for the digestion of specific carbohydrates such as cellulose
• Lactase – responsible for the digestion of lactose (Galactose + Glucose).